effects of the aromatase inhibitor letrozole on serum immunoglobulin and lysozyme levels in immunized rainbow trout (oncorhynchus mykiss walbaum) females
نویسندگان
چکیده
letrozole is a synthetic aromatase inhibitor and interfere in the committed step in the synthesis of endogenous estrogens from androgens. also estrogens regulate the immune system in teleost. changes of 17- β- esrtradiol (e 2 ), serum immunoglobulin and lysozyme levels were measured using a method based on the ability of lysozyme to lyse the bacterium micrococcus lysodeikticus , enzyme-linked immunosorbent assay (elisa) and elisa respectively. twelve broodstocks were injected weekly with 2.5 mg kg -1 letrozole (an endocrine disrupter component) two months before spawning season and vaccinated intraperitoneally (i.p) with a bacterin (inactivated l. garviae ) one month before spawning. twelve broodstocks for vaccination and twelve female rainbow trout as control group were also immiunised (i.p) with the bacterin and injected (i.p) with pbs, respectively. in the group received 2.5 mg ai kg -1 per week, serum e 2 levels were significantly lower than that of other groups. total immunoglobulin level and lysozyme activity were significantly higher in the parents received 2.5 mg kg -1 per week and were immunized with 10 -9 cells ml -1 lactococcus garvieae compared to the group which immunized with l. garvieae and the control (non- immunized). the present study, suggests that aromatase inhibitors such as letrozole may be a potential tool to regulate the synthesis of e 2 , is involved in the hormone- immune system interaction in rainbow trout.
منابع مشابه
Effects of the Aromatase Inhibitor, Letrozol, on Levels of Serum 17β-Estradiol (E2) And Enzymes in Rainbow Trout (Oncorhynchus Mykiss Walbaum) Females
In the present study, changes of values of serum 17ß-estradiol (E2), Asparate amino teransferase (AST), Alanine amino transferase (ALT) and Lactate dehydrogenase (LDH) following intraperitoneal (i.p) injection with the aromatase inhibitor (AI) letrozole were investigated in rainbow trout (Oncorhynchus mykiss). Forty- eight apparently healthy rainbow trout (mean weight of 845 g) divided...
متن کاملEffects of the Aromatase Inhibitor, Letrozol, on Levels of Serum 17β-Estradiol (E2) And Enzymes in Rainbow Trout (Oncorhynchus Mykiss Walbaum) Females
In the present study, changes of values of serum 17ß-estradiol (E2), Asparate amino teransferase (AST), Alanine amino transferase (ALT) and Lactate dehydrogenase (LDH) following intraperitoneal (i.p) injection with the aromatase inhibitor (AI) letrozole were investigated in rainbow trout (Oncorhynchus mykiss). Forty- eight apparently healthy rainbow trout (mean weight of 845 g) divided...
متن کاملEffects of letrozole, non-steroidal aromatase inhibitor, on serum sex steroid levels, oocyte diameter and gonadosomatic index (GSI) in rainbow trout (Oncorhynchus mykiss)
The objective of this study was to contribute to investigate the efficacy of letrozole, a potent non-steroidal aromatase inhibitor, on serum sex steroid levels and oocyte growth in rainbow trout females. Serum steroid levels were measured using an indirect enzyme-linked immunosorbent assay (ELISA) before and 22 days after an initial injection with 1, 2.5 mg kg-1 and subsequent weekly injection ...
متن کاملEffects of letrozole, non-steroidal aromatase inhibitor, on serum sex steroid levels, oocyte diameter and gonadosomatic index (GSI) in rainbow trout (Oncorhynchus mykiss)
The objective of this study was to contribute to investigate the efficacy of letrozole, a potent non-steroidal aromatase inhibitor, on serum sex steroid levels and oocyte growth in rainbow trout females. Serum steroid levels were measured using an indirect enzyme-linked immunosorbent assay (ELISA) before and 22 days after an initial injection with 1, 2.5 mg kg-1 and subsequent weekly injection ...
متن کاملeffects of the aromatase inhibitor, letrozol, on levels of serum 17β-estradiol (e2) and enzymes in rainbow trout (oncorhynchus mykiss walbaum) females
in the present study, changes of values of serum 17ß-estradiol (e2), asparate amino teransferase (ast), alanine amino transferase (alt) and lactate dehydrogenase (ldh) following intraperitoneal (i.p) injection with the aromatase inhibitor (ai) letrozole were investigated in rainbow trout (oncorhynchus mykiss). forty- eight apparently healthy rainbow trout (mean weight of 845 g) divided in four ...
متن کاملRainbow Trout, Oncorhynchus mykiss
Rainbow trout are classified as Oncorhynchus mykiss, and as such belong to the same genus as Pacific salmon, and to the family Salmonidae, which includes Atlantic salmon (Salmo salar), various trout (Salvelinus sp.), Arctic char (Salvelinus alpinus), Arctic grayling (Thymallus arcticus) and whitefish (Coregonus sp.). Rainbow trout are native to areas around the North Pacific Ocean, from souther...
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عنوان ژورنال:
international journal of aquatic biologyجلد ۱، شماره ۶، صفحات ۲۷۳-۰
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